Midori
Green is one of the most underrated Nucleic acid staining chemical.
It is used in the same way as ethidium bromide (EtBr), but does not
have the same disadvantages. It is used mainly in disease diagnosis
and genetic research. Do you want a replacement to your ethidium
bromide that has nothing but advantages? Get Midori
Green now!
Nucleic
Acid Stains
The
purpose of nucleic acid stains is visualizing and thus showing
results from an image of microscopic size of biological tissue. Ever
since the 1960’s, the method of Nucleic Acid Stains has been used.
There is a problem with stains, because only one in ten stains is
only reasonably hazardous for people and nature. Throughout the years
though, there have been several methods introduced that tried to be a
good replacement for the most used chemical. But, though they were
less harmful, there has never been a nucleic acid stain that has
proved to be as successful as Midori Green.
Ethidium
Bromide (EtBr)
The
most-used nucleic acid stain chemical, rather than Midori Green, is
ethidium bromide (EtBr). It is very sensitive in detecting DNA-bands
on gel. Absorption maxima can actually be seen through a microscope,
because it lies within the UV range. Small amounts of DNA can be
detected using ethidium bromide staining. Ethidium bromide can,
however, intercalate into the DNA an can interfere with replication
and transcription, it is still seen as a toxic and the use of it is
questioned, which opens doors for alternatives like Midori Green.
Advantages
The
whole goal of the development of Midori Green was to provide
advantages in relation to this current staining method, which it
does. The main concern was to make sure that it was a
non-carcinogenic and less mutagenic. Another advantage that is
provided is that it is at least as sensitive as EtBr, even small
fragments are made visible. All in all, Midori Green works great as a
nucleic acid staining method, has the same or better effect compared
to ethidium bromide and it has a lot more advantages.
